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Anti-CALD1 PHOSPHO Antibody EPR2210
Also for CALD1 (NM_033157)
|A phospho-specific peptide corresponding to residues surrounding Threonine 730 of human Caldesmon was used as an immunogen. The antibody only detects Caldesmon phosphorylated on Threonine 730.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens caldesmon 1 (CALD1), transcript variant 3|
|CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22|
Entrez Gene 800 Human
|This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. |
| Vascular smooth muscle contraction|
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Western blot - Caldesmon (phospho T730) antibody [EPR2210]; All lanes : Anti-Caldesmon (phospho T730) antibody [EPR2210] at 1/10000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 93 kDa.Observed band size : 75 kDa .