OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ARRB1 antibodies

Anti-ARRB1 Antibody E274

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303398
  • Rabbit monoclonal antibody against ß-Arrestin-1(E274)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412547) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
IF(1)
FC(1)
spacer
Also for ARRB1 (NM_020251)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-terminus of human ß-arrestin-1 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.
Clone NameE274 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC: 1:100; FC: 1:10 - 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens arrestin, beta 1 (ARRB1), transcript variant 2
Alternative NameARB1; ARR1
Database LinkNP_064647
FunctionMembers of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known. [provided by RefSeq].
Related Pathway
MAPK signaling pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - beta Arrestin 1 antibody [E274]; Anti-beta Arrestin 1 antibody [E274] at 1/500 dilution + 293 cell lysate.Predicted band size : 50 kDa.Observed band size : 50 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - beta Arrestin 1 antibody [E274]; TA303398 at a 1:100 dilution staining beta Arrestin 1 in human lymph node using Immunohistochemistry, Paraffin Embedded Tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Arrestin 1 antibody [E274]; TA303398 stainingbeta Arrestin 1in humanprostate carcinomatissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA pH9.0 and then blockingwith 1% donkey serumfor1 hourat 20°C was performed. The primary antibody was diluted 1/100and incubated with sample for1 hour at 20°C in PBS. ABiotin conjugateddonkey polyclonal to rabbit IgG was used undilutedas secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-beta Arrestin 1 antibody [E274]; TA303398 staining beta Arrestin 1 in C4-2B (Human prostate cancer cell line) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in diluent) for 1 hour at 21°C. An AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
FC Image
Flow Cytometry-beta Arrestin 1 antibody [E274](TA303398); Overlay histogram showing PC3 cells stained with TA303398 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title