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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 PHOSPHO Antibody EP2109Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303390
  • Rabbit monoclonal antibody against Akt1/PKBa (pS473) Phospho (EP2109Y ) (phospho-specific)
100ul $325 Next Day
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WB(3)
IHC(2)
IF(3)
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Also for AKT1 (NM_005163)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Serine 473.
Clone NameEP2109Y IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; WB: 1:5000 - 1:10000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_005154
FunctionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

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WB Image
Western blot - AKT1 (phospho S473) antibody [EP2109Y]; All lanes : Anti-AKT1 (phospho S473) [EP2109Y] antibody at 1/10000 dilution.Lane 1 : 3T3 cell lysate untreated.Lane 2 : 3T3 cell lysates treated with PDGF.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 56 kDa.Observed band size : 60 kDa .
WB Image
Western blot - Anti-AKT1 (phospho S473) [EP2109Y] antibody; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 56 kDa.Observed band size : 60 kDa .Exposure time : 20 minutes
WB Image
Western blot - Anti-AKT1 (phospho S473) [EP2109Y] antibody; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 56 kDa.Observed band size : 60 kDa .Exposure time : 20 minutes
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1 (phospho S473) antibody [EP2109Y]; TA303390, at 1/100 dilution, staining AKT1 in untreated (left panel) and Phosphatase-treated (right panel) cervical carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y]; Immunohistochemical analysis of Human HPV16 immortalized keratinocytes transfected with non-targeting siRNA, staining AKT1 (phospho S473) (green) with TA303390.Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Samples were blocked with 10% goat serum before incubating with primary antibody (1/100). Fluoroscein-conjugated tyramide was used to detect staining.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody [EP2109Y]; staining AKT1 (phospho S473) in PC12 cells treated with galanin (1-29) (rat, mouse) , by ICC/IF. Increase of AKT1 (phospho S473) expression correlates with increased concentration of galanin (1-29) (rat, mouse), as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (galanin (1-29) (rat, mouse)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody [EP2109Y]; staining AKT1 (phospho S473) in PC3 cells treated with CAY10626 , by ICC/IF. Decrease of AKT1 (phospho S473) expression correlates with increased concentration of CAY10626, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (CAY10626) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-AKT1 (phospho S473) antibody [EP2109Y](TA303390); TA303390 staining AKT1 (phospho S473) in MCF7 cells treated with DAPT , by ICC/IF. Decrease in expression of AKT1 (phospho S473) correlates with increased concentration of DAPT, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (DAPT) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA303390 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

 

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