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Anti-AKT1 Antibody Y89
Also for AKT1 (NM_001014432)
|A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:20; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
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Western blot - AKT1 antibody [Y89]; Anti-AKT1 antibody [Y89] at 1/10000 dilution + MCF-7 cell lysate.Predicted band size : 56 kDa.Observed band size : 59 kDa .
Immunohistochemistry (Paraffin-embedded sections) - AKT1 antibody [Y89]; Immunohistochemical analysis of paraffin-embedded prostate carcinoma using TA303387 at 1/100 dilution.
ICC/IF - Anti-AKT1 antibody; TA303387 staining AKT1 in SK-N-SH cells treated with alsterpaullone , by ICC/IF.The cells were incubated at 37°C for 6h in media containing different concentrations of (alsterpaullone) in DMSO. Staining of the treated cells with TA303387 (1/200 dilution was performed overnight at 4°C. A DyLight 488 anti-rabbit polyclonal antibody at 1:250 was used as the secondary antibody. Nuclei were counterstained with DAPI.
Flow Cytometry-AKT1 antibody(TA303387); Overlay histogram showing HeLa cells stained with TA303387 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.