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Anti-ACTC1 Antibody EP184E
Also for ACTC1 (NM_005159)
|A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actin.|
|Mouse, Rat, Cow, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1,000; IHC: 1:100 200; ICC: 1:250 500; FC: 1:20; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens actin, alpha, cardiac muscle 1 (ACTC1)|
|ACTC; ASD5; CMD1R; CMH11; LVNC4|
Entrez Gene 70 Human
Entrez Gene 11464 Mouse
Entrez Gene 29275 Rat
|Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq]. |
| Cardiac muscle contractionHypertrophic cardiomyopathy (HCM)Dilated cardiomyopathy|
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Western blot - muscle Actin antibody [EP184E]; Anti-muscle Actin antibody [EP184E] at 1/1000 dilution + A431 cell lysate at 10ug/lane.Secondary.Goat anti-rabbit, HRP labelled. at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
Immunohistochemistry (Paraffin-embedded sections) - muscle Actin antibody [EP184E]; Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin TA303378 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E]; Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using TA303378. Green-specific fluorescent staining.
Immunohistochemistry (Frozen sections) - Anti-muscle Actin antibody [EP184E]; Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with TA303378.
Immunocytochemistry/ Immunofluorescence - muscle Actin antibody [EP184E]; Immunofluorescent staining of HeLa cells using TA303378 at a 1/250 dilution.
Flow Cytometry-Anti-muscle Actin antibody(TA303378); Overlay histogram showing HeLa cells stained with TA303378 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in HeLa cells under the same conditions.