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Anti-ACTC1 Antibody EP184E
Also for ACTC1 (NM_005159)
|A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actin.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1,000; IHC: 1:100 200; ICC: 1:250 500; FC: 1:20; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens actin, alpha, cardiac muscle 1 (ACTC1)|
|ACTC; ASD5; CMD1R; CMH11; LVNC4|
|Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq]. |
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Western blot - muscle Actin antibody [EP184E]; Anti-muscle Actin antibody [EP184E] at 1/1000 dilution + A431 cell lysate at 10ug/lane.Secondary.Goat anti-rabbit, HRP labelled. at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
Immunohistochemistry (Paraffin-embedded sections) - muscle Actin antibody [EP184E]; Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin ab46805 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E]; Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.
Immunohistochemistry (Frozen sections) - Anti-muscle Actin antibody [EP184E]; Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with ab46805.
Immunocytochemistry/ Immunofluorescence - muscle Actin antibody [EP184E]; Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
Flow Cytometry-Anti-muscle Actin antibody [EP184E](ab46805); Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.