OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ACTB antibodies

Anti-ACTB Antibody EP1123Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303377
  • Rabbit monoclonal antibody against B-Actin (Cytoplasmic)(EP1123Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420311) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(2)
FC(1)
spacer
Also for ACTB (NM_001101)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.
Clone NameEP1123Y IsotypeIgG
Species ReactivityHuman, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:1000; ICC/IF: 1:50; WB: 1:500; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens actin, beta (ACTB)
Alternative NameBRWS1; PS1TP5BP1
Database LinkNP_001092
FunctionThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq].
Related Pathway
Adherens junction
Focal Adhesion

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - beta Actin antibody [EP1123Y]; All lanes : Anti-beta Actin antibody [EP1123Y] at 1/500 dilution.Lane 1 : T47D cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [EP1123Y]; TA303377 stainingbeta Actinin Mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for1 hourat room temperature; antigen retrieval was by heat mediation incitrate buffer, pH6. Samples were incubated with primary antibody (1/100 in1% FBS/BSA) for 16 hours at 4°C. An undilutedHRP-conjugatedGoat anti-rabbit IgG polyclonalwas used as the secondary antibody.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y]; Ab52614 at 1/50 dilution staining human muscle; paraffin embedded.
FC Image
Flow Cytometry - Anti-beta Actin antibody [EP1123Y]; Overlay histogram showing HeLa cells stained with TA303377 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title