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Anti-ACTB Antibody EP1123Y
Also for ACTB (NM_001101)
|A synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||FC: 1:1000; ICC/IF: 1:50; WB: 1:500; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
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Western blot - beta Actin antibody [EP1123Y]; All lanes : Anti-beta Actin antibody [EP1123Y] at 1/500 dilution.Lane 1 : T47D cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [EP1123Y]; TA303377 stainingbeta Actinin Mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for1 hourat room temperature; antigen retrieval was by heat mediation incitrate buffer, pH6. Samples were incubated with primary antibody (1/100 in1% FBS/BSA) for 16 hours at 4Â°C. An undilutedHRP-conjugatedGoat anti-rabbit IgG polyclonalwas used as the secondary antibody.
Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y]; Ab52614 at 1/50 dilution staining human muscle; paraffin embedded.
Flow Cytometry - Anti-beta Actin antibody [EP1123Y]; Overlay histogram showing HeLa cells stained with TA303377 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.