Homo sapiens erythrocyte membrane protein band 4.1-like 2 (EPB41L2), transcript variant 3
Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)
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ELISA: 1:32,000. WB: 0.03-0.1µg/ml.
erythrocyte membrane protein band 4.1 like-protein 2
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TA303296 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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