Anti-NANOG Antibody,

Also for NANOG (NM_024865)

OriGene Data

Immunogen	Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.1.
Clone Name		Isotype	Goat IgG
Species Reactivity	Human	Concentration	0.5 mg/ml
Guaranteed Application *	WB	Suggested Dilutions	ELISA: 1:64,000. WB: 0.03-0.1µg/ml. IF: 5ug/ml.
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens Nanog homeobox (NANOG)
Alternative Name	homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database Link	NP_079141
Function	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity)
Related Pathway	Wnt Signaling Pathway

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WB Image

TA303048 (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-NANOG Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data