OriGene Logo
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-GAPDH antibodies

Anti-GAPDH Antibody, Loading Control

div

Specifications Citations (3) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302944 Goat polyclonal anti-GAPDH antibody(C-terminal), Loading control 100ug $325 In Stock
LC419567 GAPDH HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart
spacer
WB(2)
IF(1)
spacer
Also for GAPDH (NM_002046)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenPeptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Clone Name IsotypeGoat IgG
Species ReactivityTest: Human, Mouse, Rat. Expected from seq similarity: Human, Mouse, Dog ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 0.01 - 0.03ug/ml, IHC: 0.3ug/ml, ICC: 0.1 - 0.3ug/ml, ELISA 1:2000
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Note In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed

Reference Data

Target Nameglyceraldehyde-3-phosphate dehydrogenase
Alternative NameG3PD|GAPD|HEL-S-162eP
Database LinkNP_002037
Entrez Gene 2597 Human
Entrez Gene 14433 Mouse
Entrez Gene 24383 Rat
Entrez Gene 403755 Dog
FunctionThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPS Glycolysis / GluconeogenesisMetabolic pathwaysAlzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
TA302944 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
grey_line

WB Image
TA302944 (0.1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
grey_line

IF Image
TA302944 (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).
grey_line

 

spacer
Inc 5000 Healthcare Company

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge