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Home Antibody All anti-GAPDH antibodies

Anti-GAPDH Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302944
  • Goat Polyclonal Antibody against GAPDH (C-terminal)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419567) , 20ug Explanation
100ug 325 In Stock
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WB(2)
IF(1)
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Also for GAPDH (NM_002046)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Clone Name IsotypeGoat IgG
Species ReactivityTest: Human, Mouse, Rat. Expected from seq similarity: Human, Mouse, Dog ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:2,000; WB: 0.01-0.03ug/ml; IHC: 0.3ug/ml; ICC: 0.1-0.3ug/ml
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Note In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed

Reference Data

Target NameHomo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1
Alternative NameG3PD; GAPD; HEL-S-162eP
Database LinkNP_002037
Entrez Gene 2597 Human
Entrez Gene 14433 Mouse
Entrez Gene 24383 Rat
Entrez Gene 403755 Dog
FunctionThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPS Glycolysis / GluconeogenesisMetabolic pathwaysAlzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
TA302944 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB Image
TA302944 (0.1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
IF Image
TA302944 (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).

 

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