Homo sapiens tripartite motif containing 5 (TRIM5), transcript variant alpha
Synonyms:
RNF88; TRIM5alpha
Immunogen
Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Human
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
ELISA: 1:16,000. WB: 1-3µg/ml. IHC: 1.0µg/ml.
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined. [provided by RefSeq].
Related Pathway
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TA302865 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302865il. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.
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