Anti-CHFR Antibody,

Also for CHFR (NM_018223)

OriGene Data

Immunogen	Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_060693.
Clone Name		Isotype	Goat IgG
Species Reactivity	Human	Concentration	0.5 mg/ml
Guaranteed Application *	IHC	Suggested Dilutions	ELISA: 1:64,000. WB: 2.5µg/ml.
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant 4
Alternative Name	RNF116; RNF196
Database Link	NP_060693
Function	E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image

TA302851 (2.5µg/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-CHFR Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data