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Home Antibody All anti-VPS35 antibodies

Anti-VPS35 Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302699 Goat Polyclonal Antibody against VPS35 / MEM3 100ug $325 In Stock
LC402657 VPS35 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)
IF(2)

OriGene Data

ImmunogenPeptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Clone Name IsotypeGoat IgG
Species ReactivityTest: Human. Expected from seq similarity: Human, Mouse, Rat, Cow Concentration0.5mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:128000. WB: 1-3µg/ml. IHC:5µg/ml
Predicted MW Explanation 91.5 kDa
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Note In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch.

Reference Data

Target NameHomo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
Alternative NameMEM3; PARK17
Database LinkNP_060676
Entrez Gene 55737 Human
Entrez Gene 65114 Mouse
Entrez Gene 25479 Rat
FunctionThis gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
TA302699 (1µg/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC Image
TA302699 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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IHC Image
TA302699 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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IF Image
TA302699 staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark, with a previous batch.
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IF Image
TA302699 staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained, with a previous batch, from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
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