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Also for FUCA2 (NM_032020)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human FUCA2.|
||WB: 1: 100~500; IHC: 1: 10~50; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens fucosidase, alpha-L- 2, plasma (FUCA2)|
|Alpha-L-fucosidase catalyzes the hydrolysis of terminal alpha-L-fucosidase linkages in glycosphingolipids and glycoproteins. At least 2 separate polymorphic alpha-L-fucosidases are recognized in man. The FUCA2 locus regulates the level of alpha-fucosidase in plasma and fibroblasts but not in leukocytes. In fucosidosis, deficiency of alpha-L-fucosidase is found in both plasma and leukocytes.|
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Western blot analysis of FUCA2 (arrow) using rabbit polyclonal FUCA2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FUCA2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with FUCA2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.