Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (PIK3CG)
Synonyms:
PI3CG; PI3K; PI3Kgamma; PIK3
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PI3KCG.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human, Mouse
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
Background
PI3KCG belongs to the pi3/pi4-kinase family of proteins. This protein is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells.
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
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(TOP LEFT) The anti-PI3KCG Pab is used in Western blot to detect PI3KCG in mouse skeletal muscle tissue lysate. (TOP RIGHT)Western blot analysis of PI3KCG (arrow) using rabbit polyclonal PI3KCG Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PI3KCG gene (Lane 2) (Origene Technologies). (BOTTOM)This data demonstrates the use of PI3KCG antibody for IHC. Frozen tissue from the mouse ear reacted with the PI3KCG antibody , which was detected by peroxidase-conjugated anti-rabbit secondary antibody and staining with Vesctain Elite ABC/DAB kit. Sections were counterstained with hematoxilin. Data and protocol kindly provided by Dr.Doinita Serban from David Cheresh Lab at UCSD.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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