This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human TAOK3.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
Background
TAOK3 is negatively regulated by epidermal growth factor (EGF) and inhibits the basal activity of Jun kinase. When TAOK3 is overexpressed, it may activate ERK1/ERK2 and JNK/SAPK.
Related Pathway
MAPK signaling pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis of TAOK3 (arrow) using rabbit polyclonal TAOK3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TAOK3 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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