This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human STK39.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human, Mouse
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1: 100~500; IHC: 1: 10~50; ELISA: 1: 1,000;
Background
STK39 is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.
Related Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
(LEFT)Western blot analysis of STK39 (arrow) using rabbit polyclonal STK39 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK39 gene (Lane 2) (Origene Technologies). (RIGHT)Western blot analysis of STK39 Antibody (C-term) polyclonal antibody in mouse liver tissue lysates (35ug/lane). STK39(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with STK39 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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