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Home Antibody All anti-PIAS1 antibodies

Anti-PIAS1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302212
  • Rabbit Polyclonal Antibody against PIAS1 (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402513) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)
IHC(1)

OriGene Data

ImmunogenThis PIAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the N-terminal region of human PIAS1.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:50~100
Predicted MW Explanation 71835 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens protein inhibitor of activated STAT, 1 (PIAS1)
Alternative NameDDXBP1; GBP; GU/RH-II; ZMIZ3
Database LinkNP_057250
Entrez Gene 8554 Human
FunctionPIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Related PathwayTranscription FactorsDruggable Genome Ubiquitin mediated proteolysisJak-STAT signaling pathwayPathways in cancerSmall cell lung cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293 cell extracts were prepared and immunoprecipitated with TA302212 (left lane alpha-PIAS1 IP) or a control antibody (right lane Control IP), followed by Western blotting with TA302212. Taken from Polyakova o, Borman S, Grimley R, Vamathevan J, Hayes B. et al. (2012) Identification of Novel interacting Partners of Sirtuin6. Plos ONE 7 (12): e51555. doi: 10.1371/journal.pone. 0051555
WB Image
Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

 

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