Homo sapiens MDM2 oncogene, E3 ubiquitin protein ligase (MDM2)
ACTFS; hdm2; HDMX
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Mdm2.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
Senescence and Autophagy
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(LEFT)Western blot analysis of anti-Mdm2 Pab in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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