OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MDM2 antibodies

Anti-MDM2 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302211
  • Rabbit Polyclonal Antibody against MDM2 (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400855) , 20ug
100ug 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)

OriGene Data

ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Mdm2.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant 1
Alternative NameACTFS; hdm2; HDMX
Database LinkNP_002383
FunctionMDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
Related Pathway
Apoptosis
Cell cycle
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
(LEFT)Western blot analysis of anti-Mdm2 Pab in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title