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Home Antibody All anti-MDM2 antibodies

Anti-MDM2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302211
  • Rabbit Polyclonal Antibody against MDM2 (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400855) , 20ug Explanation
100ug 325 In Stock
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WB(3)
IHC(1)

OriGene Data

ImmunogenThis Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 55232 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant 1
Alternative NameACTFS; hdm2; HDMX
Database LinkNP_002383
Entrez Gene 4193 Human
Entrez Gene 17246 Mouse
FunctionMDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
Related PathwayTranscription FactorsDruggable Genome Cell cyclep53 signaling pathwayUbiquitin mediated proteolysisEndocytosisPathways in cancerGliomaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) (Cat.#TA302211). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
WB Image
Western blot analysis of anti-Mdm2 Pab (Cat. #TA302211) in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
WB Image
MDM2 Antibody (Q213) (Cat.#TA302211) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MDM2 antibody detected the MDM2 protein (arrow).
IHC Image
Mdm2 Antibody (C-term) (Cat.#TA302211) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Mdm2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

 

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