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Anti-AKT2 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302205 Rabbit Polyclonal Antibody against AKT2 (S474) 100ug $325 3-7 Days
LC419836 AKT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Mouse, Rat) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:10~50, WB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 55769 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Database LinkNP_001617
Entrez Gene 208 Human
Entrez Gene 11652 Mouse
Entrez Gene 25233 Rat
FunctionAKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of C2C12 cell stained with AKT2 Antibody (S474)(Cat#TA302205).C2C12 cells were incubated with AKT2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .AKT2 immunoreactivity is localized to Cytoplasm significantly.


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