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Home Antibody All anti-AKT2 antibodies

Anti-AKT2 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA302205
  • Rabbit Polyclonal Antibody against AKT2 (S474)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419836) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenThis AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Mouse, Rat) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:10~50, WB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 55769 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Alternative NameHIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
Database LinkNP_001617
Entrez Gene 208 Human
Entrez Gene 11652 Mouse
Entrez Gene 25233 Rat
FunctionAKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474)(Cat#TA302205).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37â). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37â).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37â). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).AKT2 immunoreactivity is localized to Cytoplasm significantly.

 

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