This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y322 of human MAPK14.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Related Pathway
EGFR1 Signaling Pathway
Hemostasis
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
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(LEFT)Western blot analysis of MAPK14 Antibody (Y322) in HepG2 cell line lysates (35ug/lane). MAPK14(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of MAPK14(arrow) using rabbit polyclonal MAPK14 Antibody (Y322) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (Lane 2) (Origene Technologies).
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