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Home Antibody All anti-PIK3CA antibodies

Anti-PIK3CA Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302178
  • Rabbit Polyclonal Antibody against PIK3CA (Center)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416797) , 20ug
100ug 325 In Stock
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WB(1)
IHC(1)
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Also for PIK3CA (NM_006218)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50~100; IHC: 1:50-100; ELISA: 1: 1,000;
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA)
Alternative NameCLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K
Database LinkNP_006209
FunctionPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
(LEFT)Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution) (RIGHT)Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution).
IHC Image
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

 

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