Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA)
Synonyms:
CLOVE; MCAP; MCM; MCMTC; p110-alpha; PI3K
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; IHC: 1:50-100; ELISA: 1: 1,000;
Background
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
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(LEFT)Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution) (RIGHT)Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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