Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
B-RAF1; BRAF1; NS7; RAFB1
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human BRAF.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
BRAF, a mebmer of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
MAPK signaling pathway
Senescence and Autophagy
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Western blot analysis of BRAF Antibody (Center) polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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