This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GATA4.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1:50~100; ELISA: 1: 1,000;
GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.
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(LEFT)Western blot analysis of anti-GATA4 Antibody (C-term) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).
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