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Home Antibody All anti-NANOG antibodies

Anti-NANOG Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302154
  • Rabbit Polyclonal Antibody against NANOG (Center)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC411030) , 20ug
100ug 325 3-7 Days
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WB(1)
IHC(1)
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Also for NANOG (NM_024865)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NANOG.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Nanog homeobox (NANOG)
Alternative Namehomeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database LinkNP_079141
FunctionNANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Related Pathway
Wnt Signaling Pathway

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WB Image
(LEFT)Western blot analysis of NANOG Antibody (Center) in Hela cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

 

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