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Home Antibody All anti-NANOG antibodies

Anti-NANOG Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302154
  • Rabbit Polyclonal Antibody against NANOG (Center)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411030) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for NANOG (NM_024865)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Bovine, Monkey) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
Predicted MW Explanation 34489 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Nanog homeobox (NANOG), transcript variant 1
Alternative Namehomeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database LinkNP_079141
Entrez Gene 79923 Human
Entrez Gene 715746 Monkey
FunctionNANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Related PathwayES Cell Differentiation/IPSStem cell - PluripotencyCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cells

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
NANOG Antibody (Center) (Cat. #TA302154) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
IHC Image
Formalin-fixed and paraffin-embedded human Testis tissue reacted with NANOG antibody (Center)(Cat.#TA302154), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Immunofluorescence analysis of NANOG Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
FC Image
Flow cytometric analysis of hela cells using NANOG Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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