This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NANOG.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Related Pathway
Wnt Signaling Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis of anti-NANOG Antibody (N-term) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab (1:60 dilution). Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
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