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Home Antibody All anti-NANOG antibodies

Anti-NANOG Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302153 Rabbit Polyclonal Antibody against NANOG (N-term) 100ug $325 3-7 Days
LC411030 NANOG HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for NANOG (NM_024865)
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OriGene Data

ImmunogenThis NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Monkey) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IF: 1:10~50, IHC: 1:10~50, FC: 1:10~50
Predicted MW Explanation 34489 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Nanog homeobox (NANOG), transcript variant 1
Alternative Namehomeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database LinkNP_079141
Entrez Gene 79923 Human
Entrez Gene 715746 Monkey
FunctionNANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Related PathwayES Cell Differentiation/IPSStem cell - PluripotencyCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cells

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

IHC Image
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF Image
IF image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

IF Image
IF image of Hela cell stained with NANOG Antibody (N-term)(Cat#TA302153).Hela cells were incubated with NANOG primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NANOG immunoreactivity is localized to Nucleus significantly.

FC Image
NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


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