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[anti-HA]
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[Akt3]
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[LA]
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[JAK2]
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)
Synonyms:
ASK1; MAPKKK5; MEKK5
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S966 of human MAP3K5.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Related Pathway
MAPK signaling pathway
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Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).
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