Home Antibody All anti-JUN antibodies
|This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide HPRLQALKEEPQTVPE containing a predicted sumoylation site from the C-terminal region of human AP-1.|
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens jun proto-oncogene (JUN)|
|AP-1; AP1; c-Jun|
|The gene for AP-1 is the putative transforming gene of avian sarcoma virus 17. AP-1 is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The AP-1 gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. AP-1 dimer activity is downregulated by SUMO-1, SUMO-2, and SUMO-3.|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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(LEFT)The AP-1 C-term polyclonal antibody is used in Western blot to detect nonsumoylated AP-1 in Jurkat cell lysate. (RIGHT)Western blot analysis of AP-1(arrow) using rabbit polyclonal AP-1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AP-1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.