Home Antibody All anti-JMJD5 antibodies
Also for JMJD5 (NM_024773)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human JMJD5.|
||WB: 1:250~500; IHC: 1: 10~50; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens lysine (K)-specific demethylase 8 (KDM8), transcript variant 2|
|Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.|
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Western blot analysis of anti-JMJD5 (C-term) Pab pre-incubated with and without blocking peptide (BP1031b) in mouse liver tissue lysate. JMJD5 (C-term)(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with JMJD5 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.