This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAPK3.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Related Pathway
Adherens junction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
(LEFT) Western blot analysis of MAPK3 Antibody (N-term) Pab in HepG2 cell line lysates (35ug/lane). MAPK3(arrow) was detected using the purified polyclonal antibody. (RIGHT) Western blot analysis of MAPK3 (arrow) using purified Pab . 293 cell lysates (1 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2) (Origene Technologies).
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