Home Antibody All anti-EIF4A1 antibodies
Also for EIF4A1 (NM_001416)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EIF4A1.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1|
|DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I|
|EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.|
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(LEFT)Western blot analysis of anti-EIF4A1 Pab in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.