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Home Antibody All anti-CD9 antibodies

Anti-CD9 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA302058 Rabbit Polyclonal Antibody against CD9 (Center) 100ug $325 3-7 Days
LC400677 CD9 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(4)
IF(1)
FC(1)

OriGene Data

ImmunogenThis CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the Central region of human CD9.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1000, IF: 1:10~50, FC: 1:10~50
Predicted MW Explanation 25285 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens CD9 molecule (CD9)
Alternative NameBTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29
Database LinkNP_001760
Entrez Gene 928 Human
FunctionCD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
Related PathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsEmbryonic stem cellsDruggable Genome Hematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab.
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Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (Center) (Cat.#TA302058). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
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Western blot analysis of lysate from Hela cell line, using CD9 Antibody (Center)(Cat. #TA302058). TA302058 was diluted at 1:1000. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center) (Cat. #TA302058). TA302058 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Immunofluorescence analysis of anti-CD9 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Flow cytometric analysis of jurkat cells using CD9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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