This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CD9.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
Related Pathway
Hematopoietic cell lineage
Hemostasis
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* OriGene provides validated application data and protocol, with money back guarantee.
(LEFT)Western blot analysis of CD9 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
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