Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
Synonyms:
MAPKK2; MEK2; MKK2; PRKMK2
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T394 of human MAP2K2.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
Background
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
Toll-like receptor signaling pathway
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Western blot analysis of anti-MAP2K2 Antibody (T394) in Hela cell line lysates (35ug/lane). MAP2K2(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (T394) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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