Home Antibody All anti-ASCL1 antibodies
Also for ASCL1 (NM_004316)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Hash1.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1)|
|ASH1; bHLHa46; HASH1; MASH1|
|ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation.|
Delta-Notch Signaling Pathway
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(LEFT)The anti-Hash1 Antibody (N-term) is used in Western blot to detect Hash1 in mouse lung tissue lysate. (RIGHT)Western blot analysis of Hash1 (arrow) using rabbit polyclonal Hash1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Hash1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.