Home Antibody All anti-SOX2 antibodies
Also for SOX2 (NM_003106)
|SOX2 recombinant protein is used to produce this monoclonal antibody.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||IF: 1:100, WB: 1:200~2000, IHC: 1:50~100, FC: 1:10~50
|Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein G column, followed by dialysis against PBS.
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Western blot analysis of SOX2 Antibody (Cat.#TA302025) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.
Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#TA302025). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#TA302025), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of A549 cell stained with SOX2 Antibody(Cat#TA302025/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢).SOX2 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA302025 SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.