Homo sapiens growth factor receptor-bound protein 2 (GRB2), transcript variant 1
ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y209 of human GRB2.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
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Western blot analysis of GRB2 (arrow) using rabbit polyclonal GRB2 Antibody (Y209) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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