Anti-TP53 Antibody,

Also for TP53 (NM_000546)

OriGene Data

Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S15 of human TP53.
Clone Name		Isotype	Rabbit Ig
Species Reactivity	Human	Concentration	0.25 mg/ml
Guaranteed Application *	WB	Suggested Dilutions	WB: 1:50~100; ELISA: 1: 1,000;
Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens tumor protein p53 (TP53), transcript variant 1
Alternative Name	BCC7; LFS1; P53; TRP53
Database Link	NP_000537
Function	Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Related Pathway	Apoptosis Cell cycle Delta-Notch Signaling Pathway MAPK signaling pathway Senescence and Autophagy TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

Western blot analysis of TP53(arrow) using rabbit polyclonal p53 Antibdoy (S15) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TP53 gene (Lane 2) (Origene Technologies).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-TP53 Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data