14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a protein's function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints.
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY YWHAG (RC201925) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YWHAG.