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Home Antibody All anti-MAP1LC3B antibodies

Anti-MAP1LC3B Antibody

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA301543 Rabbit Polyclonal Antibody against LC3B 100ul $325 2 Weeks
LC402952 MAP1LC3B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(3)
IF(1)
FC(1)
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Also for MAP1LC3B (NM_022818)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:3000
BufferTris-glycine, 150mM NaCl and 0.05% sodium azide
Purification peptide affinity purified

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)
Alternative NameATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a
Database LinkNP_073729
Entrez Gene 81631 Human
Entrez Gene 67443 Mouse
FunctionAutophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates
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Figure from citation: Western Blot of MAP1LC3B protein level by using anti-MAP1LC3B antibody in both DCIS and invasive human breast cancer specimens. View Citation
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LC3B staining in treated U87-MG cultured & subcutaneous tumors
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Immunohistochemistry: LC3B Antibody - LC3B staining in gliobastoma multiform tissue.
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Immunohistochemistry: LC3B Antibody - Staining of treated U373-MG (human glioblastoma).
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Immunofluorescent staining of treated U373-MG cells. The nuclei were stained with DAPI
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Flow Cytometry: LC3B Antibody - Staining of NTERA-2 cells using NB600-1384 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary.
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