Home Antibody All anti-MAP1LC3B antibodies
Also for MAP1LC3B (NM_022818)
|A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
|Tris-glycine, 150mM NaCl and 0.05% sodium azide|
|peptide affinity purified (Protein A or G Sepharose)
|Homo sapiens microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)|
|ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a|
|Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells. |
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Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates
LC3B staining in treated U87-MG cultured & subcutaneous tumors
Immunohistochemistry: LC3B Antibody - LC3B staining in gliobastoma multiform tissue.
Immunohistochemistry: LC3B Antibody - Staining of treated U373-MG (human glioblastoma).
Immunofluorescent staining of treated U373-MG cells. The nuclei were stained with DAPI
Flow Cytometry: LC3B Antibody - Staining of NTERA-2 cells using NB600-1384 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary.