Homo sapiens hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)
Synonyms:
DKFZp762F1811; FIH1; FLJ20615; FLJ22027
Immunogen
A C-terminal region synthetic peptide made to the human FIH protein sequence (between residues 300 and the C-terminus).
Buffer
Tris-citrate/phosphate [pH 7-8] buffer. and 0.1% Sodium Azide
Clone Name
Isotype
Species Reactivity
human, rat
Concentration
1.25 mg/ml
Purification
Immunogen affinity purified
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB (1:1000-1:5000), IHC, IP
Background
Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.
Related Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY HIF1AN (RC202843) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF1AN.
