Home Antibody All anti-TARDBP antibodies
Also for TARDBP (NM_007375)
|A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein. [Swiss-Prot# Q13148]|
|human, primate, mouse, chicken, Xenopus, Zebrafish
||Lot dependent; please refer to CoA along with shipment
||WB: 0.5-2 ug/ml
|Tris-glycine, 150mM NaCl and 0.05% sodium azide|
|Immunogen affinity purified
|Homo sapiens TAR DNA binding protein (TARDBP)|
|The HIV1 virus gives rise to acquired immunodeficiency syndrome (AIDS). HIV1 contains an RNA genome which produces chromosomally integrated DNA during cellular replication. Gene activation of HIV1 by the transactivator Tat requires an RNA regulatory element TAR which is located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to the chromosomally integrated TAR DNA, preventing HIV1 expression. Additionally, this protein regulates alternative splicing of the CTFR gene, a similar pseudogene on chromosoome 20. TARDBP has also been recently been shown to abnormally accumulate in the post-mortem brain tissue of individuals diagnosed with ALS (Amyotrophic Lateral Sclerosis) and FTD (Frontal Temporal Dementia). This long-awaited conncection between these two diseases provides an explanation for the observed clinical overlap.|
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Detection of TARDBP in HeLa WCE
Western Blot: TARDBP Antibody - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.
Immunocytochemistry/Immunofluorescence: TARDBP antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).