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Also for BECN1 (NM_003766)
|NB 500-249 was raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.|
|Tris-citrate/phosphate, pH 7-8 and 0.1% sodium azide|
|Immunogen affinity purified (Protein A or G Sepharose)
|Homo sapiens beclin 1, autophagy related (BECN1)|
|ATG6; beclin1; VPS30|
|Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses. |
Senescence and Autophagy
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY BECN1 (RC201629) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
Detection of Beclin in mouse liver tissue lysate (50 ug). ECL detection at 1 minute
Pheochromocytes of the Adrenal Medulla 40x