Home Antibody All anti-BECN1 antibodies
Also for BECN1 (NM_003766)
|NB 500-249 was raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.|
|WB, IHC, IF
|Tris-citrate/phosphate, pH 7-8 and 0.1% sodium azide|
|Immunogen affinity purified (Protein A or G Sepharose)
|Homo sapiens beclin 1, autophagy related (BECN1)|
|ATG6; beclin1; VPS30|
|Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses. |
Senescence and Autophagy
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Detection of Beclin in mouse liver tissue lysate (50 ug). ECL detection at 1 minute
Western Blot: Beclin 1 Antibody - WB analysis of Belclin1. Lane 1 human brain and Lane 2 mouse brain
Pheochromocytes of the Adrenal Medulla 40x
Immunohistochemistry-Paraffin: Beclin 1 Antibody - Analysis of Beclin1 in mouse kidney.
Immunocytochemistry/Immunofluorescence: Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).