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Anti-RAD17 PHOSPHO Antibody EPR3145
Also for RAD17 (NM_133344)
|A phospho-specific peptide corresponding to residues in human Rad17 was used as an immunogen. The antibody only detects Rad17 phosphoryated on Serine 656.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5,000~10,000; IHC: 1: 100~250; ICC: 1: 100~250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 7|
|CCYC; HRAD17; R24L; RAD17SP; RAD24|
|Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between Rad17 and the checkpoint kinases, ATM and ATR (2). Rad17 has been reported to be a phosphorylation substrate of ATR and is critical for ATR-mediated checkpoint signaling and cell survival. Phosphorylation of Rad17 by ATR is important for genomic stability and restraint of S phase but is not essential for cell survival (3). |
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Western blot - Rad17 (phospho S656) antibody [EPR3145]; All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] at 1/10000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad17 (phospho S656) antibody [EPR3145]; ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Rad17 (phospho S656) antibody [EPR3145]; ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.