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Home Antibody All anti-ILK antibodies

Anti-ILK Antibody EPR1592

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301338
  • Rabbit Monoclonal Antibody against ILK (Clone EPR1592)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401438) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IF(2)
FC(1)
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Also for ILK (NM_004517)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ILK was used as an immunogen.
Clone NameEPR1592 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:5000 - 1:50000; FC: 1:100; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens integrin-linked kinase (ILK), transcript variant 1
Alternative NameHEL-S-28; ILK-1; ILK-2; P59; p59ILK
Database LinkNP_004508
Entrez Gene 3611 Human
Entrez Gene 16202 Mouse
Entrez Gene 170922 Rat
FunctionIntegrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix. Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).
Related PathwayProtein KinaseDruggable Genome PPAR signaling pathwayFocal adhesionEndometrial cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Integrin linked ILK antibody [EPR1592]; All lanes : Anti-Integrin linked ILK antibody [EPR1592] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody [EPR1592]; TA301338 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody [EPR1592]; TA301338 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-Integrin linked ILK antibody [EPR1592]; Overlay histogram showing HEK293 cells stained with TA301338 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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