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Anti-ITGA4 PHOSPHO Antibody EPR2343
Also for ITGA4 (NM_000885)
|A phospho-specific peptide corresponding to residues on human Integrin alpha 4 was used as an immunogen. The antibody only detects Integrin alpha4 phosphorylated on Serine 1027. The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 - 1:2000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)|
|Integrin alpha 4 is a cell surface heterodimer (VLA4, CD49d/CD29) which belongs to the integrin family and is involved in cell-extracellular matrix interactions, as well as in intercellular adhesion. Unlike other integrin alpha subunits, the alpha subunit of Integrin alpha 4 can appear as a 150-kDa polypeptide (alpha 150), or cleaved into two non-covalently associated polypeptides (alpha 70-80). The relative proportion of each form is highly variable among different cells and is dependent on the state of cellular activation (1). Integrin alpha 4 regulates cellular functions, differently from other integrins, through its cytoplasmic tail, making it indispensable for embryogenesis, haematopoiesis and immune responses (2). Integrin alpha 4, initially discovered on lymphoid cells, is actually known to be highly expressed on T cells, B cells, monocytes, and derived cell lines (3). It has a key function in the adhesion interaction between stem/progenitor cells and the stromal microenvironmental cells and their matrix within the bone marrow (4). |
Hematopoietic cell lineage
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Western blot - Integrin alpha 4 (phospho S1027) antibody [EPR2343]; All lanes : Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] at 1/2000 dilution.Lane 1 : THP1 cell lysates, untreated .Lane 2 : THP1 cell lysates, with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 150 kDa.Observed band size : 70,140,180 kDa .