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Anti-PRKRA Antibody EPR3224


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against PRKRA (Clone EPR3224)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401219) , 20ug Explanation
100ul $325 3-7 Days
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Also for PRKRA (NM_003690)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C terminus of human PACT was used as an immunogen.
Clone NameEPR3224 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1: 1000~5000; IHC: 1: 100~250
Predicted MW Explanation 34 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 1
Alternative NameDYT16; HSD14; PACT; RAX
Database LinkNP_003681
Entrez Gene 8575 Human
Entrez Gene 23992 Mouse
Entrez Gene 311130 Rat
FunctionProtein kinase, interferon-inducible double stranded RNA dependent activator (PACT, PRKRA) is a proapoptotic cellular protein activator of PKR, a double-stranded RNA-dependent protein kinase (1). PACT is reported to be stress-activated, and a physiologic activator that couples transmembrane stress signals and protein synthesis (2). Overexpression of PACT in cells causes enhanced sensitivity to stress-induced apoptosis. It is expressed at high levels in colonic epithelial cells, especially as they exit the cell cycle and enter an apoptotic program (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PACT (PKR activating protein) / PRKRA antibody [EPR3224]; All lanes : Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PACT (PKR activating protein) / PRKRA antibody [EPR3224]; TA301318 at 1/100 dilution staining PACT (PKR activating protein) / PRKRA in paraffin-embedded human brain tissue.


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