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Anti-PRKRA Antibody EPR3224
Also for PRKRA (NM_003690)
|A synthetic peptide corresponding to residues on the C terminus of human PACT was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1000~5000; IHC: 1: 100~250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 1|
|DYT16; HSD14; PACT; RAX|
|Protein kinase, interferon-inducible double stranded RNA dependent activator (PACT, PRKRA) is a proapoptotic cellular protein activator of PKR, a double-stranded RNA-dependent protein kinase (1). PACT is reported to be stress-activated, and a physiologic activator that couples transmembrane stress signals and protein synthesis (2). Overexpression of PACT in cells causes enhanced sensitivity to stress-induced apoptosis. It is expressed at high levels in colonic epithelial cells, especially as they exit the cell cycle and enter an apoptotic program (3). |
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Western blot - PACT (PKR activating protein) / PRKRA antibody [EPR3224]; All lanes : Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PACT (PKR activating protein) / PRKRA antibody [EPR3224]; TA301318 at 1/100 dilution staining PACT (PKR activating protein) / PRKRA in paraffin-embedded human brain tissue.