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Anti-LDHA Antibody EPR1564
Also for LDHA (NM_005566)
|A synthetic peptide corresponding to residues in human LDH-A (muscle subunit) was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250; FC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1|
|GSD11; HEL-S-133P; LDH1; LDHM; PIG19|
Entrez Gene 3939 Human
Entrez Gene 16828 Mouse
Entrez Gene 24533 Rat
|Lactate dehydrogenase (LDH) is a ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). Lactate dehydrogenase A (LDH-A, LDH muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3). |
|Druggable Genome Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways|
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Western blot - Lactate Dehydrogenase Isoenzyme V antibody; All lanes : Anti-Lactate Dehydrogenase Isoenzyme V [EPR1564] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : A375 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactate Dehydrogenase Isoenzyme V antibody; Staining of Human Lactate Dehydrogenase Isoenzyme V in a Formalin/PFA-fixed paraffin-embedded section of Human Muscle using at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564]; Immunofluorescence analysis of rat primary oligodendrocytes, staining Lactate Dehydrogenase Isoenzyme V with TA301302. Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20Â°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4Â°C. An AlexaFluor?488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.
Flow Cytometry - Anti-Lactate Dehydrogenase Isoenzyme V antibody; Overlay histogram showing HeLa cells stained with TA301302 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.