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Home Antibody All anti-LDHA antibodies

Anti-LDHA Antibody EPR1564

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301302
  • Rabbit Monoclonal Antibody against LDHA (Clone EPR1564)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401712) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for LDHA (NM_005566)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LDH-A (muscle subunit) was used as an immunogen.
Clone NameEPR1564 IsotypeIgG
Species ReactivityHuman, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250; FC: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens lactate dehydrogenase A (LDHA), transcript variant 1
Alternative NameGSD11; HEL-S-133P; LDH1; LDHM; PIG19
Database LinkNP_005557
FunctionLactate dehydrogenase (LDH) is a ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). Lactate dehydrogenase A (LDH-A, LDH muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Lactate Dehydrogenase Isoenzyme V antibody; All lanes : Anti-Lactate Dehydrogenase Isoenzyme V [EPR1564] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : A375 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 37 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactate Dehydrogenase Isoenzyme V antibody; Staining of Human Lactate Dehydrogenase Isoenzyme V in a Formalin/PFA-fixed paraffin-embedded section of Human Muscle using at a dilution of 1/500.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564]; Immunofluorescence analysis of rat primary oligodendrocytes, staining Lactate Dehydrogenase Isoenzyme V with TA301302. Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4°C. An AlexaFluor?488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-Lactate Dehydrogenase Isoenzyme V antibody; Overlay histogram showing HeLa cells stained with TA301302 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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