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Anti-CDC42 PHOSPHO Antibody EP221Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against CDC42 (Clone EP221Y) (Phospho-specific)
100ul 325 3-7 Days
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Also for CDC42 (NM_044472)
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Serine 71 of human Rac1/Cdc42 was used as an immunogen. The antibody only detects Rac1/Cdc42 phosphorylated on Serine 71.
Clone NameEP221Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500 - 1:1000; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens cell division cycle 42 (CDC42), transcript variant 2
Alternative NameCDC42Hs; G25K
Database LinkNP_426359
Entrez Gene 998 Human
FunctionRac1 and cell division cycle 42 (cdc42) are guanosine triphosphatases (GTPases). Rac1 is involved in signal transduction pathways that induce the formation of lamellipodia, stimulate cell proliferation and activate the JNK/SAPK protein kinase cascade. Rac1 regulates unique aspects of hematopoietic development and function, and is critical for the hypertrophic response in the heart. Therapies that target myocardial Rac1 are reported to be beneficial in the treatment of cardiac hypertrophy. Cdc42 has been shown in endothelial cell monolayers to prevent disassembly of interendothelial junctions and the increase in endothelial permeability. Cdc42 activity of microvessel endothelial cells is a critical determinant of junctional barrier restrictiveness and may represent a means of therapeutically modulating increased lung vascular permeability and edema formation. The phosphorylation of Rac1 inhibits its GTP-binding activity without any significant change in GTPase activity. Akt phosphorylates serine 71 of Rac1, modulating the Rac1 signal transduction pathway (1-5).
Related PathwayDruggable Genome MAPK signaling pathwayChemokine signaling pathwayEndocytosisAxon guidanceVEGF signaling pathwayFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RAC1 + Cdc42 (phospho S71) antibody [EP221Y]; All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] at 1/1000 dilution.Lane 1 : A431 cell lysate treated with Lambda Phosphatase.Lane 2 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Observed band size : 24 kDa .
IF Image
Immunocytochemistry - RAC1 + Cdc42 (phospho S71) antibody [EP221Y]; Immunohistochemical analysis of A431-2 using TA301301 at a 1/100 dilution.


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