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Anti-CDC42 PHOSPHO Antibody EP221Y
Also for CDC42 (NM_044472)
|A phospho-specific peptide corresponding to residues surrounding Serine 71 of human Rac1/Cdc42 was used as an immunogen. The antibody only detects Rac1/Cdc42 phosphorylated on Serine 71.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500 - 1:1000; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens cell division cycle 42 (CDC42), transcript variant 2|
Entrez Gene 998 Human
|Rac1 and cell division cycle 42 (cdc42) are guanosine triphosphatases (GTPases). Rac1 is involved in signal transduction pathways that induce the formation of lamellipodia, stimulate cell proliferation and activate the JNK/SAPK protein kinase cascade. Rac1 regulates unique aspects of hematopoietic development and function, and is critical for the hypertrophic response in the heart. Therapies that target myocardial Rac1 are reported to be beneficial in the treatment of cardiac hypertrophy. Cdc42 has been shown in endothelial cell monolayers to prevent disassembly of interendothelial junctions and the increase in endothelial permeability. Cdc42 activity of microvessel endothelial cells is a critical determinant of junctional barrier restrictiveness and may represent a means of therapeutically modulating increased lung vascular permeability and edema formation. The phosphorylation of Rac1 inhibits its GTP-binding activity without any significant change in GTPase activity. Akt phosphorylates serine 71 of Rac1, modulating the Rac1 signal transduction pathway (1-5). |
|Druggable Genome MAPK signaling pathwayChemokine signaling pathwayEndocytosisAxon guidanceVEGF signaling pathwayFocal adhesionMore Pathways >> |
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Western blot - RAC1 + Cdc42 (phospho S71) antibody [EP221Y]; All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] at 1/1000 dilution.Lane 1 : A431 cell lysate treated with Lambda Phosphatase.Lane 2 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Observed band size : 24 kDa .
Immunocytochemistry - RAC1 + Cdc42 (phospho S71) antibody [EP221Y]; Immunohistochemical analysis of A431-2 using TA301301 at a 1/100 dilution.