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Anti-PEBP1 Antibody EPR2875Y
Also for PEBP1 (NM_002567)
|A synthetic peptide corresponding to residues in human RKIP was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||ICC: 1:100; IHC-P: 1:250 - 1:500; IHC-Fr: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; IP: 1:10; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens phosphatidylethanolamine binding protein 1 (PEBP1)|
|HCNP; HCNPpp; HEL-210; HEL-S-34; PBP; PEBP; PEBP-1; RKIP|
Entrez Gene 5037 Human
Entrez Gene 23980 Mouse
Entrez Gene 29542 Rat
|Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine-binding protein (PEBP) family. It is an inhibitor of the Raf/MEK/MAP kinase signaling cascade and is a suppressor of cancer metastasis (1). RKIP is involved in neural development, cardiac function and spermatogenesis and appears to have serine protease activity. The inhibition Raf-1-mediated phosphorylation of MEK results from RKIP binding to Raf-1. Dysregulated RKIP expression has the potential to contribute to pathophysiological processes including Alzheimer's disease and diabetic nephropathy (2). RKIP represents a novel effector of signal transduction pathways leading to apoptosis and a prognostic marker of the pathogenesis of human cancer cells and tumors (3). |
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Western blot - PBP antibody [EPR2875Y]; All lanes : Anti-PBP antibody [EPR2875Y] at 1/5000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A549 cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 21 kDa.Observed band size : 21 kDa.
Western blot - Anti-PBP antibody [EPR2875Y]; Anti-PBP antibody [EPR2875Y] at 1/5000 dilution + Human PBP full length protein at 0.1 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBP antibody [EPR2875Y]; Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 TA301294.