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Home Antibody All anti-PEBP1 antibodies

Anti-PEBP1 PHOSPHO Antibody EP2845Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA301282
  • Rabbit Monoclonal Antibody against PEBP1 (Clone EP2845Y) (Phospho-specific)
100ul $325 In Stock

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WB(1)
IHC(1)
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Also for PEBP1 (NM_002567)
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding serine 153 of human RKIP was used as an immunogen. This antibody detects RKIP phosphorylated on serine 153.
Clone NameEP2845Y IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens phosphatidylethanolamine binding protein 1 (PEBP1)
Alternative NameHCNP; HCNPpp; HEL-210; HEL-S-34; PBP; PEBP; PEBP-1; RKIP
Database LinkNP_002558
Entrez Gene 5037 Human
Entrez Gene 29542 Rat
FunctionRaf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine-binding protein (PEBP) family. It is an inhibitor of the Raf/MEK/MAP kinase signaling cascade and is a suppressor of cancer metastasis (1). RKIP is involved in neural development, cardiac function and spermatogenesis and appears to have serine protease activity. The inhibition Raf-1-mediated phosphorylation of MEK results from RKIP binding to Raf-1. Dysregulated RKIP expression has the potential to contribute to pathophysiological processes including Alzheimer's disease and diabetic nephropathy (2). RKIP represents a novel effector of signal transduction pathways leading to apoptosis and a prognostic marker of the pathogenesis of human cancer cells and tumors (3). RKIP is a physiological inhibitor of GRK-2. After stimulation of GPCR, RKIP dissociates from its known target, Raf-1 (refs 6-8), to associate with GRK-2 and block its activity. This switch is triggered by protein kinase C (PKC)-dependent phosphorylation of the RKIP on serine 153 (4).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PBP (phospho S153) antibody [EP2845Y]; All lanes : Anti-PBP (phospho S153) antibody [EP2845Y] at 1/2000 dilution.Lane 1 : HeLa cell lysates un-treated.Lane 2 : HeLa cell lysates treated with calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 21 kDa.Observed band size : 21 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBP (phospho S153) antibody [EP2845Y]; TA301282, at 1/100 dilution, staining PBP in human liver by immunohistochemistry using paraffin-embedded tissue.
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